• Full name of the gene, NCBI # or Uniprot ID.
• If possible, provide us the full sequence of the gene (optional).
• Expected application: ELISA? WB? IHC? ICC? IF? Flow Cyto?
• What sample the antibody plan to detect? Please indicated this in detail, e.g. A431 cell lysate, mouse lung tissue, etc.
• Other requirements: like need tag at the sequence; just need anti-serum? Manufacture according to the customers' sequence, etc.

• Analyze the sequence, design and sythesize the peptides (2 different peptides or more to ensure the success, 15-20aa, if need longer, please indicate, price will be increased for more than 20aa).
• Conjugated the peptides with the carrier, usually the KLH or BSA
• Immunize rabbits (usually at least 4 rabbits), mouse, or goat with the peptides (Multiple immunization for each rabbit, mouse or goat), during this period, we need collect the sample and test to determined the immunization.
• Collect the anti-serum after immunization and test.
• Purify the antibody from the anti-serum if the results are good (if the testing results are not good, we will immunize again).

• ELISA (1:20000-100000) with high stability & sensitivity.
• The expected application with the same or the similar samples of customers.

• 75 days (maybe prolonged or decreased for different antibodies)

• Purified antibody, 10-50mg. (For goat polyclonal, will be greater than 100mg)
• All the rest anti-serum (End user can use this for testing or purifying the antibody) or the ascites for mouse polyclonal, 10ml. (For goat polyclonal, will be greater than 100ml)
• Rest peptide / immunogen, 3mg.
• Report for the whole service & manual

• Rabbit polyclonal : Please inquiry
• Mouse polyclonal : Please inquiry
• Goat polyclonal : Please inquiry

• If customer can provide the peptide (requirements: volume: 3-5mg for each gene, purity > 85%), price will be cheaper.
• If customer just need anti-serum, not the purified antibodies, the price will be different, please inquiry and indicate at the beginning.
• We respect the intellectual property of the antibody, will not sell it commercially unless get the permit from customer.

Please note : The above information is for custom polyclonal antibodies which used the synthesized peptide as the immunogen, we also provide the custom polyclonal antibodies service which used the recombinant protein as immunogen, please inquiry for the details.

• Full name of the gene, NCBI # or Uniprot ID.
• If possible, provide us the full sequence of the gene (optional).
• Expected application: ELISA? WB? IHC? ICC? IF? Flow Cyto?
• What sample the antibody plan to detect? Please indicated this in detail, e.g. A431 cell lysate, mouse lung tissue, etc.
• Other requirements: like need tag at the sequence; just need anti-serum? Manufacture according to the customers' sequence, etc.

• Analyze the sequence, design and synthesize the peptide. (15-20aa, for longer, the price will be increased).
• Conjugated the peptides with the carrier, usually the KLH or BSA.
• Immunize mouse (usually 5 Balb/c mouses) with the peptides.
• Cell fusion
• Screen the best positive clone.
• Hybridoma cloning.
• Freeze and recovery the hybridoma cells.
• Culture in vitro to get cell culture supernatants, inoculate in vivo to get ascites.
• Purify the ascites to get antibody and test.

• ELISA (1:20000-100000) with high stability & sensitivity.
• The expected application with the same or the similar samples of customers.

• 150 days (maybe prolonged or decreased for different antibodies)

• ≥3 hybridoma cells.
• Purified antibody, 5mg-10mg.
• Report for the whole service & manual

• Please inquiry

• If customer can provide the peptide (requirements: volume: 3-5mg for each gene, purity > 85%).
• If customer just need ascites, not the purified antibodies, the price will be different, please inquiry and indicate at the beginning.
• We respect the intellectual property of the antibody, will not sell it commercially unless get the permit from customer.

Please note : The above information is for custom monoclonal antibodies which used the synthesized peptide as the immunogen, we also provide the custom monoclonal antibodies service which used the recombinant protein as immunogen, please inquiry for the details.