| OLIGOS SYNTHESIS | |
|---|---|
| DNA Oligos - Tube & Plate Format | RNA Oligos - Tube Format |
| Dual Labbled DNA Probes | Chimaric Oligos |
| Custom DNA Genes | Long Oligos & Extra Long Oligos |
| DNA & RNA Modified Oligos | Ready Made Primer |
| DNA & RNA Purified Oligos | Duplex Oligos |
| Dual Labeled Probes | Molecular Beacons |
| CUSTOM DNA OLIGOS TUBE & PLATE FORMAT | ||
|---|---|---|
| SCALE | LENGTH | YIELD * |
| 25 nmol | 15-60 Bases | 3 OD’s |
| 50 nmol | 10-60 Bases | 3 OD’s |
| 100 nmol | 10-90 Bases | 6 OD’s |
| 250 nmol | 5-100 Bases | 15 OD’s |
| 1 μmol | 5-100 Bases | 45 OD’s |
| 5 μmol | 5-60 Bases | 225 OD’s |
| 10 μmol | 5-60 Bases | 450 OD’s |
•Yields are listed unmodified oligos will vary
| Custom RNA Oligos Chimeric DNA Bases 2’-O Methyl RNA Bases Phosphorothioate Bonds DNA & RNA 5 Methyl dC Bases |
Scale | Yield * |
|---|---|---|
| 100 nmol | 4.5 OD’s | |
| 250 nmol | 11.3 OD’s | |
| 1 μmol | 33.8 OD’s | |
| 5 μmol | Inquire | |
| 10 μmol | Inquire |
Yields are listed unmodified oligos will vary
| PURIFICATIONS DNA OLIGOS | |||||
| Scale | Types of Purification | ||||
| 100 nmol | PAGE | HPLC | RNase Free HPLC | Dual HPLC | Dual HPLC & PAGE |
| 250 nmol | PAGE | HPLC | RNase Free HPLC | Dual HPLC | Dual HPLC & PAGE |
| 1 μmol | PAGE | HPLC | RNase Free HPLC | Dual HPLC | Dual HPLC & PAGE |
| 5 μmol | PAGE | HPLC | RNase Free HPLC | Dual HPLC | Dual HPLC & PAGE |
| 10 μmol | PAGE | HPLC | RNase Free HPLC | Dual HPLC | Dual HPLC & PAGE |
| Long Oliogs 60 - 200 Bases Modification Available for Long Oligoes |
Scale | Purification | Yield * |
| 4-nmol Tube Format | Standard Desalted | 4 nmol | |
| 20-nmol Tube Format | Standard Desalted | 20 nmol | |
| 20-nmol Tube Format | PAGE Purification | Inquire | |
| 4-nmol Plate Format | Standard Desalted | 4nmol/well | |
| 20-nmol Plate Format | Standard Desalted | 20nmol/well | |
| We are delivering Long Oligos normalized and lyophilized in Tube & Plate Format | |||
| Ideal for Gene Construction, Cloning and ddRNAi | |||
| Extra Long Oligos Upto 500 Bases |
|
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| ReadyMade™ Primer Name | Sequence | Tm | Anhyd. Mol. Wgt. | pmoles 10µgs |
|---|---|---|---|---|
| 16S rRNA For | AGA GTT TGA TCC TGG CTC AG | 55.2 | 6148.0 | 1626.7 |
| 16S rRNA Rev | ACG GCT ACC TTG TTA CGA CTT | 57.4 | 6372.2 | 1569.4 |
| 3' RACE PCR | GGC CAC GCG TCG ACT AGT AC | 60.6 | 6103.0 | 1638.5 |
| Anchored Oligo dT (20) | TTT TTT TTT TTT TTT TTT TV | 39.2 | 6028.3 | 1658.7 |
| Anchored Oligo dT (22) | TTT TTT TTT TTT TTT TTT TTV N | 42.8 | 6641.4 | 1505.7 |
| BGH Reverse | TAG AAG GCA CAG TCG AGG | 54.0 | 5597.7 | 1786.4 |
| Bluescript KS | TCG AGG TCG ACG GTA TC | 53.3 | 5226.4 | 1913.4 |
| Bluescript SK | CGC TCT AGA ACT AGT GGA TC | 52.4 | 6117.0 | 1634.8 |
| cDNA Cloning Primer | GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TV | 64.8 | 11584.9 | 863.1 |
| EGFP-C | CAT GGT CCT GCT GGA GTT CGT G | 61.2 | 6773.4 | 1476.3 |
| EGFP-N | CGT CGC CGT CCA GCT CGA CCA G | 67.2 | 6657.3 | 1502.1 |
| GAPDH For | ACC ACA GTC CAT GCC ATC AC | 58.6 | 5991.0 | 1669.3 |
| GAPDH Rev | TCC ACC ACC CTG TTG CTG TA | 59.7 | 6003.9 | 1665.7 |
| M13 Forward (-20) | GTA AAA CGA CGG CCA GT | 53.0 | 5228.5 | 1912.6 |
| M13 Forward (-41) | CGC CAG GGT TTT CCC AGT CAC GAC | 65.5 | 7289.8 | 1371.7 |
| M13 Reverse (-27) | CAG GAA ACA GCT ATG AC | 47.3 | 5212.5 | 1918.3 |
| M13 Reverse (-48) | AGC GGA TAA CAA TTT CAC ACA GG | 57.2 | 7065.7 | 1415.2 |
| Neomycin For | CTT GGG TGG AGA GGC TAT TC | 55.6 | 6204.1 | 1612.0 |
| Neomycin Rev | AGG TGA GAT GAC AGG AGA TC | 54.0 | 6255.1 | 1598.7 |
| Oligo dT, 15mer | TTT TTT TTT TTT TTT | 29.7 | 4501.0 | 2221.7 |
| Oligo dT, 16mer | TTT TTT TTT TTT TTT T | 32.1 | 4805.2 | 2081.0 |
| Oligo dT, 18mer | TTT TTT TTT TTT TTT TTT | 36.0 | 5413.6 | 1847.3 |
| Oligo dT, 20mer | TTT TTT TTT TTT TTT TTT TT | 39.1 | 6022.0 | 1660.6 |
| Oligo dT, 20mer w/ 5' Phos | TTT TTT TTT TTT TTT TTT TT | 39.1 | 6101.9 | 1638.8 |
| PCMV Forward | CGC AAA TGG GCG GTA GGC GTG | 64.8 | 6552.3 | 1526.2 |
| pET 3' | CTA GTT ATT GCT CAG CGG | 50.6 | 5505.6 | 1816.2 |
| pET 5' (T7) | TAA TAC GAC TCA CTA TAG G | 45.3 | 5795.8 | 1725.3 |
| pET Upstream | ATG CGT CCG GCG TAG A | 56.7 | 4922.2 | 2031.4 |
| pGEX 3' | CCG GGA GCT GCA TGT GTC AGA GG | 65.2 | 7145.7 | 1399.3 |
| pGEX 5' | GGG CTG GCA AGC CAC GTT TGG TG | 67.0 | 7136.6 | 1401.1 |
| Random Hexamer | NNN NNN | <10 | 1791.7 | 5581.3 |
| Random Hexamer w/ Biotin | /5Bio/NNN NNN | <10 | 2198.2 | 4549.3 |
| SP6 Promoter | TAC GAT TTA GGT GAC ACT ATA G | 50.0 | 6773.5 | 1476.2 |
| SP6 Upstream | ATT TAG GTG ACA CTA TAG | 42.8 | 5537.7 | 1805.8 |
| T3 Promoter | AAT TAA CCC TCA CTA AAG GG | 50.4 | 6094.0 | 1640.9 |
| T7 Promoter | TAA TAC GAC TCA CTA TAG GG | 48.3 | 6125.0 | 1632.5 |
| T7 Terminator | GCT AGT TAT TGC TCA GCG G | 54.1 | 5834.8 | 1713.8 |